Anti-Human CD3&STEAP1 Reference Antibody (Xaluritamig, RUO)

Catalog No.	HV409026
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-scFv-CH/G1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	PRSS24, STEAP1, STEAP, Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1, T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact the lab for this information.
Expression system	Mammalian Cells
Accession	Q9UHE8 & P07766
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	2559056-68-5
Note	For research use only. Not suitable for clinical or therapeutic use.

SDS-PAGE	SEC-HPLC detection for Research Grade Xaluritamig.
Flow Cytometry	Flow-cytometry using anti-human STEAP1 antibody. LNCAP cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human STEAP1 monoclonal antibody (Catalog HV409026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E monoclonal antibody (Catalog HV409026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Xaluritamig.