Research Grade Cetuximab

Catalog No.	HF004026
Host species	Chimeric
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact the lab for this information.
Expression system	Mammalian Cells
Accession	P00533
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name	FabC225, IMC-225, cetuximab-IR700, 205923-56-4
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	SEC-HPLC detection for Research Grade Cetuximab.
SDS-PAGE	SDS-PAGE for Research Grade Cetuximab.
Flow CytoMetry	Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: HF004026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: HF004026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: HF004026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity	Detects recombinant EGFR in indirect ELISAs.