Recombinant Streptococcus pyogenes Cas9 Protein, N-His

카탈로그 번호	JN045012
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
순도	>90% as determined by SDS-PAGE.
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
내독소 수준	Please contact with the lab for this information.
발현 시스템	E. coli
등록번호	Q99ZW2
단백질 길이	Met1-Asp1368
성질	Recombinant
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
재구성	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name	CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046
종	Streptococcus pyogenes serotype M1
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
예측 분자량	170.39 kDa
참고사항	For research use only.