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Recombinant Human CD262/TNFRSF10B/DR5 Protein, C-His
概要
カタログ番号 HT348021
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
純度 >90% as determined by SDS-PAGE.
アプリケーション ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
エンドトキシンレベル Please contact with the lab for this information.
発現システム Mammalian Cells
アクセッション番号 O14763
タンパク質長 Ser53-Glu182
性質 Recombinant
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
再構成 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186
Homo sapiens (Human)
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
予測分子量 17.46 kDa
注意事項 For research use only.
説明

Recombinant Human CD262/TNFRSF10B/DR5 Protein, C-His (HT348021) expressed in Mammalian Cells, spanning Ser53-Glu182. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • Mammalian Expression — Proper folding and native glycosylation.
  • High Purity — >90% purity verified by SDS-PAGE.

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SDS-PAGE
SDS-PAGE for Recombinant Human CD262/TNFRSF10B/DR5 Protein, C-His

Bioactivity
Detects Eftozanermin Alfa in indirect ELISAs.

Bioactivity
Detects TAS266 in indirect ELISAs.

Bioactivity
Detected by Research Grade Benufutamab (Cat No.: HT348016) in indirect ELISA.

Western blot
Detected by Anti-CD262/TNFRSF10B/DR5 monoclonal antibody (Catalog No: HT296046)

Bioactivity
Detected by Aplitabart (Catalog No: HT348176) in indirect ELISA.