Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -172°C for twelve months from the date of receipt.
Western blot analysis was performed using anti-PCNA polyclonal antibody at 1ug/mL on various samples. Lane 1: NIH3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: Hela cell lysate Lane 4: MCF-7 cell lysate Lane 5: HepG2 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lane 8: Mouse spleen lysate