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Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966)
Overview
Catalog No. HF526066
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target TNFSF15, Vascular endothelial cell growth inhibitor, VEGI, TL1, TNF ligand-related molecule 1, Tumor necrosis factor ligand superfamily member 15, TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact the lab for this information.
Expression system Mammalian Cells
Accession O95150 & P01375
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name Bispecific, AMG966, AMG-966
Clone ID AMG 966
Note For research use only. Not suitable for clinical or therapeutic use.
Description

Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966) (HF526066) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.

Images


SDS-PAGE		 SDS-PAGE for Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966).

Bioactivity		 Detects recombinant human TNFa (Cat No.: HF879011) in indirect ELISA.

Bioactivity		 Detects recombinant human TNFa (Cat No.: HF526011) in indirect ELISA.

Bioactivity		 Detects Recombinant Human TNFSF15/TL1A Protein, C-His (Cat No.: HF526011) in indirect ELISA.

Flow CytoMetry		 Flow-cytometry APC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry FITC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF526066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot		 Western blot analysis was performed using anti-TNFSF15/TL1A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human TNFSF15/TL1A (Catalog No: HF526011)
Lane 2: negative control