Recombinant MERS-CoV nsp3 Protein, N-His

Catalog No.	VK072032
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Purity	>90% as determined by SDS-PAGE.
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level	Please contact with the lab for this information.
Expression system	E. coli
Accession	K9N638
Protein length	Gln1481-Gly1811(Lys1573Gly, Ile1717Leu)
Nature	Recombinant
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Alternative name	Replicase polyprotein 1a, pp1a, ORF1a polyprotein, Host translation inhibitor nsp1, nsp1, Leader protein, Non-structural protein 2, nsp2, p65 homolog, Papain-like protease nsp3, PL-PRO, EC:3.4.19.12, EC:3.4.22.-, Non-structural protein 3, nsp3, PL2-PRO, Non-structural protein 4, nsp4, 3C-like proteinase nsp5, 3CL-PRO, 3CLp, EC:3.4.22.69, nsp5, Non-structural protein 6, nsp6, Non-structural protein 7, nsp7, Non-structural protein 8, nsp8, RNA-capping enzyme subunit nsp9, Non-structural protein 9, nsp9, EC:2.7.7.50, Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Non-structural protein 11, nsp11
Species	Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV)
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Predicted molecular weight	39.16 kDa
Note	For research use only