| Catalog No. | VK085012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q66578 |
| Protein length | Gly111-Asn289 |
| Nature | Recombinant |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Alternative name | Genome polyprotein, Capsid protein VP0, P1AB, Virion protein 0, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, Protein 2A H-NC, P2A, Protein 2A, Protein 2B, P2B, Protein 2C, P2C, 3.6.1.15, Protein 3A, P3A, Viral protein genome-linked, VPg, Protein 3B, P3B, Protease 3C, P3C, 3.4.22.28, Picornain 3C, RNA-directed RNA polymerase 3D-POL, P3D-POL, 2.7.7.48 |
| Species | Human parechovirus 1 (HPeV-1) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Predicted molecular weight | 22.40 kDa |
| Note | For research use only |
Recombinant HPeV-1 VP0 Protein, N-His
Overview
Description
Recombinant HPeV-1 VP0 Protein, N-His (VK085012) expressed in E. coli, spanning Gly111-Asn289. Purity: >90% by SDS-PAGE.
Highlights
- ●His-Tagged — N-terminal 6×His tag for IMAC purification.
- ●E. coli Expression — High-yield, cost-effective production.
- ●High Purity — >90% purity verified by SDS-PAGE.
Images
 SDS-PAGE |
SDS-PAGE for Recombinant HPeV-1 VP0 Protein, N-His |