Anti-Human MUC16/CA125 Antibody (SAA0160)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Target
Mucin-16, CA-125, Ovarian carcinoma antigen CA125, CA125, MUC-16, Ovarian cancer-related tumor marker CA125, MUC16
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
SEC-HPLC
SEC-HPLC detection for Anti-Human MUC16/CA125 Antibody (ABS1166).
Bioactivity
Detects MUC16/CA125 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human MUC16 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MUC16 antibody monoclonal antibody (Catalog # HV601207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human MUC16 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MUC16 antibody monoclonal antibody (Catalog # HV601207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.