| Catalog No. | HY583107 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | FCM |
| Target | sIL6R, Interleukin-6 receptor subunit alpha, CD126, IL-6R 1, IL-6R subunit alpha, Membrane glycoprotein 80, gp80, IL6R, IL-6RA, IL-6 receptor subunit alpha, IL-6R-alpha |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P08887 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | SAA0048 |
| Note | For research use only. |
Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048)
Overview
Description
Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048) [SAA0048] (HY583107) is a mouse monoclonal antibody detecting IL-6RA in FCM. Suitable for Human.
Highlights
- ●Flow Cytometry — Validated for flow cytometry applications.
Images
 Flow Cytometry |
Flow-cytometry using anti-human CD126 antibody.CD126 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CD126 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD126 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 monoclonal antibody (Catalog: HY583107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CD126/IL6RA monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD126/IL6RA (Catalog No: HY583011) Lane 2: negative control |