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Anti-Human CD126/IL6R/IL-6RA Antibody (SAA0048)
Overview
Catalog No. HY583107
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM
Target sIL6R, Interleukin-6 receptor subunit alpha, CD126, IL-6R 1, IL-6R subunit alpha, Membrane glycoprotein 80, gp80, IL6R, IL-6RA, IL-6 receptor subunit alpha, IL-6R-alpha
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P08887
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDSAA0048
Note For research use only.
Images


Flow Cytometry
Flow-cytometry using anti-human CD126 antibody.CD126 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human CD126 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using anti-human CD126 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 monoclonal antibody (Catalog: HY583107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-CD126/IL6RA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD126/IL6RA (Catalog No: HY583011)
Lane 2: negative control