Anti-Human CD146/MUC18/MCAM Antibody (C3.19.1)

Catalog No.	HW484107
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG2a, kappa
Applications	FCM
Target	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P43121
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	C3.19.1
Note	For research use only.

Flow Cytometry	Flow-cytometry using anti-human CD146 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # HW484107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human CD146 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # HW484107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-CD146/MCAM monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD146/MCAM (Catalog No: HW484011) Lane 2: negative control