Anti-Human CD18/ITGB2 Antibody (SAA0815)
Overview
Catalog No.
HY378107
Host species
Human
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1
Applications
ELISA, FCM, IHC, WB
Target
Complement receptor C3 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Integrin beta-2, MFI7, CD18, ITGB2, RHC13101
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P05107
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA0815
Note
For research use only.
Images
Flow-cytometry
Flow-cytometry using anti- human CD18 antibody.
Human U-937cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Human U-937cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti- human CD18 antibody.
Human Jurkat cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Human Jurkat cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity
SEC-HPLC detection for Anti-Human CD18/ITGB2 Antibody (ABS0955).
Flow CytoMetry
Flow-cytometry using anti-human CD18 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD18 monoclonal antibody (Catalog: HY378107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.