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Anti-Human CD185/CXCR5 Antibody (16D7)
Overview
Catalog No. HB160107
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM
Target CXC-R5, CD185, MDR-15, C-X-C chemokine receptor type 5, CXCR5, CXCR-5, MDR15, Monocyte-derived receptor 15, Burkitt lymphoma receptor 1, BLR1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P32302
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID 16D7
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human CD185/CXCR5 Antibody (16D7).

Flow Cytometry
Flow-cytometry using anti-Human CD185/CXCR5 antibody. 293T cells transfected with CD185/CXCR5 (Red Histogram) or Empty vector (Yellow Histogram) with an anti-human CD185/CXCR5 antibody monoclonal antibody (Catalog #HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF647 conjugated Goat anti-Mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using anti-human CD185 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD185 monoclonal antibody (Catalog: HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.