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HEK293T Human CD268/BAFFR Stable Cell Line
Overview
Catalog No. HV599818
Brand abinScience
Host species HEK293T
Specifications 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Target CD268/BAFFR
Accession Q96RJ3
Stability and Storage Frozen in liquid nitrogen.
Properties Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Species Human
Product Usage Information
Application Dilution
Culture Medium DMEM, 10% FBS, 1% P/S
Freeze Medium FBS:DMSO=9:1
Description

The HEK293T/Human BAFFR Stable Cell Line was engineered to express the full length human BAFFR (Uniprot ID: Q96RJ3). Surface expression of human BAFFR was confirmed by flow cytometry.

Images


Flow cytometry
Flow cytometry validation of a BAFFR stable cell line using Ianalumab-APC. Human BAFFR-stable cells were incubated with APC-labeled Ianalumab (5 µg/ml) for 30 minutes at room temperature. After washing, cells were analyzed on a NovoCyte Flow Cytometer. APC-positive signal (yellow histogram) indicates specific binding of Ianalumab to BAFFR, whereas unstained cells (blue histogram) serve as a negative control, confirming surface expression of human BAFFR.