Anti-Human CD22 Antibody (RFB-4)
Overview
Catalog No.
HB011307
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1, kappa
Applications
ELISA, FCM
Target
Sialic acid-binding Ig-like lectin 2, Siglec-2, CD22, T-cell surface antigen Leu-14, BL-CAM, SIGLEC2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22, RFB4, RFB 4
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P20273
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
RFB-4
Note
For research use only.
Images
Bioactivity
Detects CD22 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human CD22 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD22 antibody monoclonal antibody (Catalog # HB011307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.