Anti-Human CD22 Antibody (RFB-4)
Overview
| Catalog No. |
HB011307 |
| Host species |
Mouse |
| Species reactivity |
Human |
| Form |
Liquid |
| Storage buffer |
0.01M PBS, pH 7.4. |
| Purity |
>95% as determined by SDS-PAGE. |
| Clonality |
Monoclonal |
| Isotype |
IgG1, kappa |
| Applications |
ELISA, FCM |
| Target |
Sialic acid-binding Ig-like lectin 2, Siglec-2, CD22, T-cell surface antigen Leu-14, BL-CAM, SIGLEC2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22, RFB4, RFB 4 |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Endotoxin level |
Please contact with the lab for this information. |
| Accession |
P20273 |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID |
RFB-4 |
| Note |
For research use only. |
Images
 Bioactivity |
Detects CD22 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CD22 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD22 antibody monoclonal antibody (Catalog # HB011307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |