| Catalog No. | HT139107 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | FCM |
| Target | BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | O00481 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Alternative name | hu103.2 |
| Clone ID | 103.2# |
| Note | For research use only. |
Anti-Human CD277/BTN3A1 Antibody (103.2#)
Overview
Images
 Bioactivity |
Detects CD277/BTN3A1 in indirect ELISAs. |
 Flow-cytometry |
Flow-cytometry using anti-human CD277 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD277 antibody monoclonal antibody (Catalog # HT139107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human CD277/BTN3A1 Antibody (103.2#) |
 Western blot |
Western blot analysis was performed using anti-CD277/BTN3A1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD277/BTN3A1 (Catalog No: HT139011) Lane 2: negative control |