| Catalog No. | HC341107 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | FCM |
| Target | Tetherin, Bone marrow stromal antigen 2, BST2, BST-2, CD317, HM1.24 antigen |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q10589 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | HM1.24 |
| Note | For research use only. |
Anti-Human BST-2/Tetherin Recombinant Antibody (HM1.24)
Overview
Images
 Bioactivity |
Detects CD317/BST2 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CD317 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD317 antibody monoclonal antibody (Catalog # HC341107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CD317 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD317 antibody monoclonal antibody (Catalog # HC341107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CD317 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD317 (Catalog No: HC341011) Lane 2: negative control |