Anti-Human CD318/CDCP1 Antibody (CUB4)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Target
CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
Bioactivity
Detects Human CD318/CDCP1 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human CD318 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 antibody monoclonal antibody (Catalog # HV451107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human CD318 antibody.SW480 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 antibody monoclonal antibody (Catalog # HV451107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.