Anti-Human CD3E Antibody (OKT3)
Overview
Catalog No.
HY057307
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
FCM
Target
T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P07766
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
OKT3
Note
For research use only.
Images
Bioactivity
SEC-HPLC detection for Anti-Human CD3E Antibody (OKT3).
Flow Cytometry
Flow-cytometry using anti-human CD3E antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # HY057307 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human CD3E antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # HY057307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.