Anti-Human CD50/ICAM3 Antibody (SAA1382)
Overview
Catalog No.
HW443107
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1
Applications
FCM
Target
CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P32942
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA1382
Note
For research use only.
Images
Bioactivity
Detects Human CD50/ICAM3 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human CD50 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 antibody monoclonal antibody (Catalog # HW443107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human CD50 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 antibody monoclonal antibody (Catalog # HW443107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.