| Catalog No. | HB677107 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | FCM |
| Target | MIC2Y, E2 antigen, MIC2X, 12E7, Protein MIC2, CD99 antigen, MIC2, T-cell surface glycoprotein E2, CD99 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P14209 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | 12E7# |
| Note | For research use only. |
Anti-Human CD99 Antibody (12E7#)
Overview
Description
Anti-Human CD99 Antibody (12E7#) [12E7#] (HB677107) is a mouse monoclonal antibody detecting CD99 in FCM. Suitable for Human.
Highlights
- ●Flow Cytometry — Validated for flow cytometry applications.
Images
 Bioactivity |
Detects CD99 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CD99 antibody.CD99 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CD99 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CD99 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |