Research Grade Bimekizumab

Catalog No.	HS856036
Host species	Humanized
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17A, IL17A, CTLA8, IL-17, Interleukin-17A, IL17F, Interleukin-17F, IL-17F, Cytokine ML-1
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Expression system	Mammalian Cells
Accession	Q16552 & Q96PD4
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative name	CDP4940, UCB-4940, UCB4940, 1418205-77-2
Species	Human
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	Detects IL17F in indirect ELISAs.
SEC-HPLC	SEC-HPLC detection for Research Grade Bimekizumab.
Bioactivity	Detects Human IL17A in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human IL17A&IL17F antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL17A&IL17F monoclonal antibody (Catalog HS856036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Bimekizumab.
Western blot	Western blot analysis was performed using anti-IL17A monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL17A (Catalog No: HS856011), 50ng Lane 2: recombinant human IL17A (Catalog No: HS856011), 30ng Lane 3: recombinant human IL17A (Catalog No: HS856011), 20ng Lane 4: recombinant human IL17A (Catalog No: HS856011), 10ng