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Anti-Mouse CD16/CD32 Antibody (2.4G2)
Overview
Catalog No.
MP369107
Host species
Rat
Species reactivity
Mouse
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Applications
FCM, FuncS, IF, IP
Target
Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P08508, P08101
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
2.4G2
Note
For research use only.
Images

Bioactivity

Detects CD16/CD32 in indirect ELISAs.

Flow Cytometry

Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.