Anti-Human HLA-B*27 Antibody (H4H2)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Target
HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, HLAB, HLA-B*27
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
Flow Cytometry
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # HM814107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.