Anti-Human IL1A/IL1F1 Antibody (SAA0366)

Catalog No.	HF802107
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1, kappa
Applications	ELISA, FCM
Target	IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P01583
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	SAA0366
Note	For research use only.

Flow-cytometry	Flow-cytometry using anti-human IL1A antibody.IL1A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry	Flow-cytometry using anti-human IL1A antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry	Flow-cytometry using anti-human IL1A antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.