データシートをダウンロード
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
概要
カタログ番号 HF879107
宿主種 Mouse
種反応性 Human
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 0.09% Sodium azide.
純度 >95% as determined by SDS-PAGE.
クローン性 Monoclonal
アイソタイプ IgG2a, kappa
アプリケーション ELISA, FCM
ターゲット TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
精製 Protein A/G purified from cell culture supernatant.
エンドトキシンレベル Please contact with the lab for this information.
アクセッション番号 P01375
安定性と保存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
クローンID SAA0415
注意事項 For research use only.
説明

Anti-Human TNFa/TNF-alpha Antibody (SAA0415) [SAA0415] (HF879107) is a mouse monoclonal antibody detecting TNF-alpha in ELISA, FCM. Suitable for Human.
Highlights
  • ●Flow Cytometry — Validated for flow cytometry applications.

イメージ


Flow-cytometry		 Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry		 Flow-cytometry using anti-human TNFa antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.