Anti-Human OPN/SPP1 Antibody (SAA0512)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Applications
ELISA, FCM, SPR, WB
Target
Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512)
Flow Cytometry
Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.