| Catalog No. | HY207107 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | ELISA, FCM, SPR, WB |
| Target | Osteopontin, BNSP, Uropontin, OPN, SPP-1, Secreted phosphoprotein 1, Bone sialoprotein 1, Nephropontin, Urinary stone protein, SPP1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P10451 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | SAA0512 |
| Note | For research use only. |
Anti-Human OPN/SPP1 Antibody (SAA0512)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-Human OPN/SPP1 Antibody (SAA0512) |
 Flow Cytometry |
Flow-cytometry using anti-human OPN antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human OPN/SPP1 (Catalog No: HY207012) Lane 2: negative control |