Anti-Human CD172a/SIRPA Antibody (SAA1393)
Overview
Catalog No.
HX892207
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1, kappa
Applications
FCM
Target
BIT, Signal-regulatory protein alpha-1, Brain Ig-like molecule with tyrosine-based activation motifs, MFR, SIRP, Sirp-alpha-1, Sirp-alpha-2, PTPNS1, SIRPA, SHP substrate 1, CD172 antigen-like family member A, Sirp-alpha-3, Inhibitory receptor SHPS-1, Signal-regulatory protein alpha-3, CD172a, Tyrosine-protein phosphatase non-receptor type substrate 1, MyD-1 antigen, Bit, SHPS-1, Macrophage fusion receptor, Signal-regulatory protein alpha-2, SHPS1, MYD1, p84
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P78324
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA1393
Note
For research use only.
Images
Bioactivity
Detects CD172a/SIRPA in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human CD172a antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a antibody monoclonal antibody (Catalog # HX892207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human CD172a antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a antibody monoclonal antibody (Catalog # HX892207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using anti-human CD172a antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD172a monoclonal antibody (Catalog: HX892207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.