Anti-Human CD71/TFRC Antibody (SAA1396)

Catalog No.	HY036207
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG2a, kappa
Applications	FCM
Target	Transferrin receptor protein 1, TfR1, T9, Trfr, p90, CD71, TFRC, TfR, sTfR, TR
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P02786
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	SAA1396
Note	For research use only.

Flow-cytometry	Flow-cytometry using anti-human CD71 antibody.CD71 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry	Flow-cytometry using anti-human CD71 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 monoclonal antibody (Catalog: HY036207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.