Anti-Human CD262/TNFRSF10B/TRAIL-R2 Antibody (SAA1413)
Overview
Catalog No.
HT348307
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, lambda
Applications
FCM
Target
TRAILR2, TRAIL-R2, Death receptor 5, CD262, DR5, Tumor necrosis factor receptor superfamily member 10B, ZTNFR9, TRAIL receptor 2, TRICK2, TNFRSF10B, KILLER, TNF-related apoptosis-inducing ligand receptor 2
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
O14763
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA1413
Note
For research use only.
Images
Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.CD262 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity
SEC-HPLC detection for Anti-Human CD262/TNFRSF10B/TRAIL-R2 Antibody (ABS1916).
Flow CytoMetry
Flow-cytometry using anti-human CD262 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD262 monoclonal antibody (Catalog: HT348307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.