Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21)
Overview
Catalog No.
HY286307
Host species
Mouse
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity
>95% as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
ELISA, FCM, IHC, WB
Target
p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
P04626
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
A21
Note
For research use only.
Images
Flow-cytometry
Flow-cytometry using anti-human ERBB2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry
Flow-cytometry using anti-human ERBB2 antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.