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Anti-Human FGF19 Antibody (SAA1435)
Overview
Catalog No. HF862013
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1
Applications ELISA, FCM, IHC, WB
Target FGF19, Fibroblast growth factor 19, FGF-19
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession O95750
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDSAA1435
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human FGF19 Antibody (ABS0484).

Flow Cytometry
Flow-cytometry using anti-human FGF19 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF19 antibody monoclonal antibody (Catalog # HF862013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Human FGF19 Antibody (SAA1435).

Western blot
Western blot analysis was performed using anti-human FGF19 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human FGF19 (Catalog No: HF862012)
Lane 2: negative control