Anti-Human IL1RL1/ST2 antibody (STLM208)
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% as determined by SDS-PAGE.
Applications
ELISA, FCM, WB
Target
T1, DER4, Interleukin-1 receptor-like 1, ST2, IL1RL1, Protein ST2
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note
For research use only.
Images
SEC-HPLC
SEC-HPLC detection for Anti-Human IL1RL1/ST2 antibody (STLM208).
Bioactivity
Detects IL1RL1/ST2 in indirect ELISAs.
Flow Cytometry
Flow-cytometry using anti-human IL1RL1 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1RL1 antibody monoclonal antibody (Catalog # HX139033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human IL1RL1 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1RL1 antibody monoclonal antibody (Catalog # HX139033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human IL1RL1/ST2 antibody (STLM208).