Anti-Human CD33 Antibody (P67.6), PE
Overview
Storage buffer
0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
Target
Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Endotoxin level
Please contact with the lab for this information.
Stability and Storage
Store at 4°C for 12 months. Protect from light. Do not freeze.
Note
For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
Flow Cytometry
Flow-cytometry using PE anti-human CD33 antibody. RMPI-8226 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD33 antibody monoclonal antibody (Catalog # HB185127, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using PE anti-human CD33 antibody. HL-60 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD33 antibody monoclonal antibody (Catalog # HB185127, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.