Anti-Human FOXP3 Monoclonal Antibody (1A209)

Catalog No.	HV388025
Host species	Mouse
Species reactivity	Human
Immunogen	Recombinant Human FOXP3 protein.
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Clonality	Monoclonal
Isotype	IgG1
Applications	ELISA, FCM, WB
Target	Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2
Purification	Protein A/G purified from cell culture supernatant.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	1A209
Note	For research use only.

Flow Cytometry	Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.