Anti-CD4 Polyclonal Antibody

Overview

Catalog No.

HF998014

Host species

Rabbit

Species reactivity

Human, Cercocebus atys, Chlorocebus aethiops, Erythrocebus patas, Macaca fascicularis, Macaca fuscata fuscata, Macaca mulatta, Macaca nemestrina, Pan troglodytes

Immunogen

E. coli - derived recombinant Human CD4 (Lys26-Trp390).

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Target

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4

Purification

Purified by antigen affinity column.

Accession

P01730

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Images

Immunofluorescence

HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD4 (HF998014) at a concentration of 10.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CD4 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).