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Anti-CKMT1A Polyclonal Antibody
Overview
Catalog No. HY409014
Host species Rabbit
Species reactivity Bos taurus, Chicken, Human, Mouse, Rat, Pig
Immunogen E. coli - derived recombinant Human CKMT1A (Ser41-Ile250).
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality Polyclonal
Isotype IgG
Applications ELISA, IHC, WB
Target U-MtCK, CKMT1A, CKMT, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, Mia-CK, Creatine kinase U-type, mitochondrial
Purification Purified by antigen affinity column.
Accession P12532
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Product Usage Information
ApplicationDilution
ELISA1:5000-1:20000
IHC1:50-1:500
WB1:500-1:2000
Note For research use only.
Images


Immunofluorescence
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CKMT1A (HY409014) at a concentration of 12.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CKMT1A was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).