Anti-CKMT1A Polyclonal Antibody
Overview
Catalog No. |
HY409014 |
Host species |
Rabbit |
Species reactivity |
Bos taurus, Chicken, Human, Mouse, Rat, Pig |
Immunogen |
E. coli - derived recombinant Human CKMT1A (Ser41-Ile250). |
Form |
Liquid |
Storage buffer |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
Clonality |
Polyclonal |
Isotype |
IgG |
Applications |
ELISA, IHC, WB |
Target |
U-MtCK, CKMT1A, CKMT, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, Mia-CK, Creatine kinase U-type, mitochondrial |
Purification |
Purified by antigen affinity column. |
Accession |
P12532 |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
Product Usage Information |
Application | Dilution |
---|
ELISA | 1:5000-1:20000 | IHC | 1:50-1:500 | WB | 1:500-1:2000 |
|
Note |
For research use only. |
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CKMT1A (HY409014) at a concentration of 12.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CKMT1A was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |