Anti-E1B-55K/E1B-495R Polyclonal Antibody (VK466014)

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Prix(USD) : $

Spécification :

50ug
100ug

Nombre :

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Aperçu

Numéro de catalogue

VK466014

Description

Anti-E1B-55K/E1B-495R Polyclonal Antibody (VK466014) is a rabbit polyclonal antibody detecting E1B-495R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human adenovirus C serotype 2, Human adenovirus C serotype 5

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Isotype

IgG

Clonalité

Polyclonal

Immunogène

E. coli - derived recombinant HAdV-5 E1B-55K/E1B-495R (Met1-Gln79).

Cible

E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R

Purification

Purified by antigen affinity column.

Numéro d'accès

P03243

RRID

Anti-E1B-55K/E1B-495R Polyclonal Antibody (abinScience Cat# VK466014, RRID:AB_3730219)

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E1B 55 kDa protein (E1B-55K) is a ~55 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.

1. Harada, JN. et al. (2002) Journal of virology 76, 9194-206. PMID: 12186903

2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032

3. Schwartz, RA. et al. (2008) Journal of virology 82, 9043-55. PMID: 18614635

4. Schreiner, S. et al. (2010) Journal of virology 84, 7029-38. PMID: 20484509

5. Pennella, MA. et al. (2010) Journal of virology 84, 12210-25. PMID: 20861261

6. Wimmer, P. et al. (2016) Oncogene 35, 69-82. PMID: 25772236

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-E1B-55K/E1B-495R polyclonal antibody at 1ug/mL on recombinant E1B-55K/E1B-495R (Catalog No. VK466012).
Western blot

Western blot analysis was performed using anti-E1B polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HAdV-5 E1B-55K/E1B-495R (Catalog No: VK466012)
Western blot

Western blot analysis was performed using anti-E1B polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HAdV-5 E1B-55K/E1B-495R (Catalog No: VK466012)