Anti-MAPK14 Polyclonal Antibody (HS804014) is a rabbit polyclonal antibody detecting MAPK14 in ELISA, IHC, WB. Suitable for Dog, Cyprinus carpio, Danio rerio, Human, and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Reactividad de especies
Dog, Cyprinus carpio, Danio rerio, Human, Mouse, Pan troglodytes, Rat, Xenopus laevis
Aplicaciones
ELISA, IHC, WB
Especie huésped
Rabbit
Isotipo
IgG
Clonalidad
Polyclonal
Inmunógeno
E. coli - derived recombinant Human MAPK14 (Met1-Ser360).
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación
Dilución
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo
Mitogen-activated protein kinase 14 (MAPK14) is a ~41 kDa protein. Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1.
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2. Pierrat, B. et al. (1998) The Journal of biological chemistry 273, 29661-71. PMID: 9792677
3. Scheper, GC. et al. (2001) Molecular and cellular biology 21, 743-54. PMID: 11154262
4. Sayed, M. et al. (2000) The Journal of biological chemistry 275, 16569-73. PMID: 10747897
5. Khurana, A. et al. (2006) The Journal of biological chemistry 281, 35316-26. PMID: 17003045
6. Webber, JL. et al. (2010) The EMBO journal 29, 27-40. PMID: 19893488
7. Zwang, Y. et al. (2006) The EMBO journal 25, 4195-206. PMID: 16932740
8. Xu, P. et al. (2010) Molecular cell 37, 551-66. PMID: 20188673
Nota
For research use only.
Imágenes
Western blot
Western blot analysis was performed using anti-MAPK14 polyclonal antibody at 1ug/mL on recombinant MAPK14 (Catalog No. HS804012).
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