Anti-Human RPS3 Polyclonal Antibody (HB915014)

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Spécification :

50ug
100ug

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Reférences
Fiche technique

Aperçu

Numéro de catalogue

HB915014

Description

Anti-Human RPS3 Polyclonal Antibody (HB915014) is a rabbit polyclonal antibody detecting RPS3 in ELISA, IHC, WB. Suitable for Ambystoma mexicanum, Bovine, Dog, Human, and Ictalurus punctatus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Ambystoma mexicanum, Arabidopsis thaliana, Bos taurus, Dog, Drosophila melanogaster, Human, Ictalurus punctatus, Manduca sexta, Mouse, Oryctolagus cuniculus, Pongo abelii, Rat, Pig, Xenopus laevis

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Isotype

IgG

Clonalité

Polyclonal

Immunogène

E. coli - derived recombinant Human RPS3 (Met1-Lys187).

Cible

Small ribosomal subunit protein uS3, RPS3, 40S ribosomal protein S3

Purification

Purified by antigen affinity column.

Numéro d'accès

P23396

RRID

Anti-Human RPS3 Polyclonal Antibody (abinScience Cat# HB915014, RRID:AB_3730678)

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Small ribosomal subunit protein uS3 (RPS3) is a ~26 kDa protein. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS).

1. Anger, AM. et al. (2013) Nature 497, 80-5. PMID: 23636399

2. Vladimirov, SN. et al. (1996) European journal of biochemistry 239, 144-9. PMID: 8706699

3. Kim, J. et al. (1995) The Journal of biological chemistry 270, 13620-9. PMID: 7775413

4. Kim, SH. et al. (2005) Biochemical and biophysical research communications 328, 962-7. PMID: 15707971

5. Hegde, V. et al. (2004) DNA repair 3, 121-6. PMID: 14706345

6. Balyeva, KE. et al. (2008) Molekuliarnaia biologiia 42, 314-22. PMID: 18610840

7. Hegde, V. et al. (2004) Biochemistry 43, 14211-7. PMID: 15518571

8. Ko, SI. et al. (2008) Mutation research 648, 54-64. PMID: 18973764

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RPS3 polyclonal antibody at 1ug/mL on recombinant Human RPS3 (Catalog No. HB915012).

Reférences

Fiche technique

Anti-Human RPS3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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