Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

Name: Anti-CD247/CD3Z Polyclonal Antibody
Brand: abinScience
SKU: HB150014
Price: 96 USD
Availability: InStock

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Precio(USD): $

Especificación:

20ug
50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet

Vista general

Número de catálogo

HB150014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Isotipo

IgG

Clonalidad

Polyclonal

Inmunógeno

E. coli - derived recombinant Human CD247/CD3Z (Arg52-Arg164).

Objetivo

CD3Z, CD247, TCRZ, T-cell receptor T3 zeta chain, T3Z, T-cell surface glycoprotein CD3 zeta chain

Purificación

Purified by antigen affinity column.

Número de acceso

P20963

RRID

Anti-CD247/CD3Z Polyclonal Antibody (abinScience Cat# HB150014, RRID:AB_3722950)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

T-cell surface glycoprotein CD3 zeta chain (CD247/CD3Z) is a ~18 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.

1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049

2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Iwashima, M. et al. (1994) Science (New York, N.Y.) 263, 1136-9. PMID: 7509083

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CD247 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

Referencias

Datasheet

Anti-CD247/CD3Z Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo