Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014)

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50ug
100ug

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Numéro de catalogue

VK475014

Description

Anti-ASFV UBC/pI215L Polyclonal Antibody (VK475014) is a rabbit polyclonal antibody detecting pI215L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

African swine fever virus

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Isotype

IgG

Clonalité

Polyclonal

Immunogène

E. coli - derived recombinant ASFV UBC/pI215L (Met1-Thr200).

Cible

Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L

Purification

Purified by antigen affinity column.

Numéro d'accès

P27949

RRID

Anti-ASFV UBC/pI215L Polyclonal Antibody (abinScience Cat# VK475014, RRID:AB_3724852)

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin-conjugating enzyme E2 (UBC/pI215L) is a ~24 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication. May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery. Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling.

1. Freitas, FB. et al. (2018) Scientific reports 8, 3471. PMID: 29472632

2. Barrado-Gil, L. et al. (2020) Frontiers in microbiology 11, 622907. PMID: 33384682

3. Barrado-Gil, L. et al. (2021) Viruses 13. PMID: 34204411

4. Huang, L. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 2754-2769. PMID: 34759016

5. Hingamp, PM. et al. (1995) Journal of virology 69, 1785-93. PMID: 7853518

Note

For research use only.

Images

Reférences

Fiche technique

Anti-ASFV UBC/pI215L Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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