Anti-Human GPC3 Antibody (SAA2001) (HW310043)

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20ug
50ug
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1mg

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Numéro de catalogue

HW310043

Description

Anti-Human GPC3 Antibody (SAA2001) [SAA2001] (HW310043) is a human monoclonal antibody detecting GPC3 in ELISA, WB. Suitable for Human.

Reactivité des espèces

Human

Applications

ELISA, WB

Espèce hôte

Human

Isotype

IgG4, kappa

ID de clone

SAA2001

Clonalité

Monoclonal

Cible

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P51654

RRID

Anti-Human GPC3 Antibody (SAA2001) (abinScience Cat# HW310043, RRID:AB_3727913)

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human GPC3 Antibody (SAA2001)
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate