Anti-BRCC3 Polyclonal Antibody (HA695014)

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Prix(USD) : $

Spécification :

50ug
100ug

Nombre :

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Reférences
Fiche technique

Aperçu

Numéro de catalogue

HA695014

Description

Anti-BRCC3 Polyclonal Antibody (HA695014) is a rabbit polyclonal antibody detecting Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53 in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Bovine

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Isotype

IgG

Clonalité

Polyclonal

Immunogène

E. coli - derived recombinant human BRCC3 (Val12-Thr223).

Cible

Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P46736, A5PJP6

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Lys-63-specific deubiquitinase BRCC36 is a ~36 kDa protein. Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates.

1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193

2. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690

3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

4. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097

5. Dong, Y. et al. (2003) Molecular cell 12, 1087-99. PMID: 14636569

6. Chen, X. et al. (2006) Cancer research 66, 5039-46. PMID: 16707425

7. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341

9. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746

10. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-BRCC3 polyclonal antibody at 1ug/mL on recombinant BRCC3 (Catalog No. HA695012).

Reférences

Fiche technique

Anti-BRCC3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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