Anti-ENO1 Polyclonal Antibody (HY081014) is a rabbit polyclonal antibody detecting ENO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anas platyrhynchos, and Bovine.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Reactividad de especies
Human, Mouse, Rat, Alligator mississippiensis, Anas platyrhynchos, Bos taurus, Chicken, Macaca fascicularis, Pongo abelii, Python regius, Sceloporus undulatus, Thunnus albacares, Trachemys scripta elegans
Aplicaciones
ELISA, IHC, WB
Especie huésped
Rabbit
Isotipo
IgG
Clonalidad
Polyclonal
Inmunógeno
E. coli - derived recombinant Human ENO1 (Pro267-Leu354).
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación
Dilución
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo
Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209
2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581
3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057
4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133
5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901
Nota
For research use only.
Imágenes
Western blot
Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on recombinant ENO1 (Catalog No. HY081012).
Western blot
Western blot analysis was performed using anti-ENO1 polyclonal antibody at 1ug/mL on various samples. Lane 1: Hela cell lysate Lane 2: LO2 cell lysate Lane 3: PC-3 cell lysate Lane 4: SGC-7901 cell lysate Lane 5: MCF-7 cell lysate Lane 6: Mouse lung lysate Lane 7: Mouse skeletal muscle lysate Lane 8: Mouse brain lysate Lane 9: Rat lung lysate Lane 10: Rat skeletal muscle lysate Lane11: Rat brain lysate
中文
English
한국어
日本語
Español
Français
Русский
