Anti-CD152/CTLA4 Polyclonal Antibody (HB651014)

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Prix(USD) : $

Spécification :

50ug
100ug

Nombre :

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Reférences
Fiche technique

Aperçu

Numéro de catalogue

HB651014

Description

Anti-CD152/CTLA4 Polyclonal Antibody (HB651014) is a rabbit polyclonal antibody detecting CD152 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Isotype

IgG

Clonalité

Polyclonal

Immunogène

E. coli - derived recombinant Human CD152/CTLA4 (Ala37-Asp161).

Cible

CD152, Cytotoxic T-lymphocyte protein 4, CTLA-4, CTLA4, Cytotoxic T-lymphocyte-associated antigen 4

Purification

Purified by antigen affinity column.

Numéro d'accès

P16410

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Cytotoxic T-lymphocyte protein 4 (CD152/CTLA4) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. CD152 is the therapeutic target of ipilimumab (Yervoy).

1. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501

2. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502

3. Teft, WA. et al. (2006) Annual review of immunology 24, 65-97. PMID: 16551244

4. Linsley, PS. et al. (1991) The Journal of experimental medicine 174, 561-9. PMID: 1714933

5. Zheng, Y. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1683-91. PMID: 18641304

6. Ramagopal, UA. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E4223-E4232. PMID: 28484017

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD152/CTLA4 polyclonal antibody at 1ug/mL on recombinant CD152/CTLA4 (Catalog No. HB651012).

Reférences

Fiche technique

Anti-CD152/CTLA4 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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