Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Reactividad de especies
Cyprinus carpio, Danio rerio, Chicken, Human, Mouse, Rat, Xenopus laevis
Aplicaciones
ELISA, IHC, WB
Especie huésped
Rabbit
Isotipo
IgG
Clonalidad
Polyclonal
Inmunógeno
E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).
Objetivo
MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación
Dilución
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo
Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.
1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315
2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637
5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239
6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871
7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800
8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730
9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304
10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692
Nota
For research use only.
Imágenes
Western blot
Western blot analysis was performed using anti-MAPK8 polyclonal antibody at 1ug/mL on recombinant MAPK8 (Catalog No. HW707012).
Western blot
Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples. Lane 1: Hela cell lysate
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