Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Reactivité des espèces
Cyprinus carpio, Danio rerio, Chicken, Human, Mouse, Rat, Xenopus laevis
Applications
ELISA, IHC, WB
Espèce hôte
Rabbit
Isotype
IgG
Clonalité
Polyclonal
Immunogène
E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).
Cible
MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte
Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.
1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315
2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637
5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239
6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871
7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800
8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730
9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304
10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692
Note
For research use only.
Images
Western blot
Western blot analysis was performed using anti-MAPK8 polyclonal antibody at 1ug/mL on recombinant MAPK8 (Catalog No. HW707012).
Western blot
Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples. Lane 1: Hela cell lysate
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