Anti-Human MET/c-Met/HGFR Antibody (224G11) (HY196207)

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Каталожный номер	HY196207
Описание	Anti-Human MET/c-Met/HGFR Antibody (224G11) [224G11] (HY196207) is a human monoclonal antibody detecting MET in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Human
Изотип	IgG1, kappa
ID клона	224G11
Клоональность	Monoclonal
Цель	Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P08581
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	Hepatocyte growth factor receptor (MET/HGFR) is a ~155 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. MET is the therapeutic target of amivantamab (Rybrevant).
Примечание	For research use only.

Изображения

Flow Cytometry

Flow-cytometry using anti-human c-Met antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog # HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human c-Met antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog # HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human c-Met antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human c-Met antibody monoclonal antibody (Catalog # HY196207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.