Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3) (HF004207)

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Datasheet

Vista general

Número de catálogo	HF004207
Descripción	Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3) [E7.6.3] (HF004207) is a human monoclonal antibody detecting ERBB1 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Human
Isotipo	IgG2, kappa
ID de clon	E7.6.3
Clonalidad	Monoclonal
Objetivo	HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P00533
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix). 1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725 2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536 3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960 4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049 6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079 7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704
Nota	For research use only.

Imágenes

Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3).pdf

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